Cat.No. : CSC-RT0232
Host Cell: 293T Target Gene: UBE2D3
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0232 |
| Description | 293T-UBE2D3 (-/-) is a stable cell line with a homozygous knockout of human UBE2D3 |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Target Gene | UBE2D3 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
As I continue to employ UBE2D3 Knockout Cell Line-293T in my experiments, I am eager to delve deeper into the complexities of genetics, unearthing new insights and uncharted territories.
Beyond the confines of my lab, I recognize the potential of UBE2D3 Knockout Cell Line-293T to contribute to advancements in medical research and therapeutic development, offering hope for future breakthroughs.
The knowledge gained through using UBE2D3 Knockout Cell Line-293T has not only enriched my academic journey but has also ignited a sense of responsibility to contribute meaningfully to society through my research.
As I collaborate with fellow scientists and exchange experiences with UBE2D3 Knockout Cell Line-293T, I am reminded of the power of shared knowledge and the collaborative spirit that drives scientific progress.
With every gene knockout experiment, I am reminded of the privilege and responsibility that comes with being a part of the scientific community, committed to advancing our understanding of the world through the lens of genetics.
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