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TPM3 Knockout Cell Line-293T

TPM3 Knockout Cell Line-293T

Cat.No. :  CSC-RT0337

Host Cell:  293T Target Gene:  TPM3

Size:  1x10^6 cells/vial, 1mL Validation:  Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0337
Description 293T-TPM3 (-/-) is a stable cell line with a homozygous knockout of human TPM3
Target Gene TPM3
Host Cell 293T
Host Cell Species Homo sapiens (Human)
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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Background

Publications

Q & A

Customer Reviews

This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Propels

With TPM3 Knockout Cell Line-293T's effectiveness, this gene knockout reagent propels my genetic exploration with unwavering reliability.

United States

08/23/2020

Accelerates

TPM3 Knockout Cell Line-293T's simplicity accelerates progress across various domains, from medical research to biotechnology.

United States

05/12/2021

Consistency

TPM3 Knockout Cell Line-293T's consistency builds confidence, underpinning the significance of my scientific inquiries.

United Kingdom

08/15/2021

Validate

TPM3 Knockout Cell Line-293T's reliable outcomes validate the precision of gene editing, enriching my research contributions.

United States

10/09/2021

Efficiency

TPM3 Knockout Cell Line-293T embodies efficiency, guiding me through complex genetic investigations.

United States

01/27/2022

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