Cat.No. : AD00399Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00399Z |
| Description | Human Adenovirus Type5 (dE1/E3) expressing Toll Interacting Protein under CMV promoter. No fusion tag, pre-made adenovirus, ready to ship and ready to use format. |
| Target Gene | TOLLIP |
| Product Type | Adenoviral particle |
| Insert | TOLLIP |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | Toll Interacting Protein |
| Gene Symbol | TOLLIP |
| Gene ID | 54472 |
| mRNA Refseq | BC018272 |
Numerous studies have shown that microRNA (miR) modification onto MSCs prevents cell death or autophagy, thereby limiting the size of MI and improving myocardial repair in MI tissue. miRs are considered reliable biomarkers of MI because they regulate downstream genes or axes and affect myocardial function. miR-324 is downregulated in hypoxia/reoxygenation-induced injured cardiomyocytes and inhibits cell proliferation. Here, it was shown that adipose-derived stem cell (ADSC) transplantation modestly enhanced cardiac function in MI rats, reduced enzyme levels, and reduced infarct size and apoptosis; whereas miR-324-5p-modified ADSCs could better promote repair after MI. Mechanistically, miR-324-5p targets TOLLIP in myocardial tissue. Furthermore, TOLLIP overexpression attenuated the promoting effect of miR-324-5p-modified ADSCs in repair after myocardial infarction (MI) in rats. These results suggest that miR-324-5p-modified ADSCs significantly enhance myocardial repair after MI by targeting TOLLIP in myocardial tissue.
To confirm that miR-324-5p-modified ADSCs promote myocardial repair by targeting TOLLIP in myocardial tissue, the researchers conducted a functional rescue experiment. By injecting adenovirus (Ad)-TOLLIP into the miR-ADSCs group rats, the expression of TOLLIP in myocardial tissue was successfully upregulated (Figure 1A). The results showed that the myocardial function of MI rats was weakened (Figure 1B), and the levels of cTnT and CK-MB in serum were increased (Figure 1C). In addition, after overexpression of TOLLIP, the degree of MI and apoptotic cells in the myocardial tissue of MI rats were significantly increased (Figures 1D and 1E). These results indicate that TOLLIP overexpression disrupts the promoting effect of miR-324-5p-modified ADSCs in post-MI repair in rats.
Figure 1. TOLLIP overexpression spoils the promotive role of miR-324-5p-modified ADSCs in post-MI repair in rats. (Ji Z, et al., 2021)
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This adenovirus helped us dissect TOLLIP’s role in inflammation with precision. High infection efficiency even in primary macrophages.
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