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Cat.No. : CSC-RT0100
Host Cell: K562 Target Gene: TMEM187
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0100 |
| Description | K562-TMEM187 cell line is a stable cell line with a homozygous knockout of TMEM187 |
| Target Gene | TMEM187 |
| Host Cell | K562 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
TMEM187 Knockout Cell Line-K562 has exceeded my expectations in every aspect. Its exceptional knockout efficiency and reliable performance have provided me with a solid foundation for studying gene function and exploring novel therapeutic targets.
United States
07/19/2022
I am truly impressed with the versatility of TMEM187 Knockout Cell Line-K562. It has consistently delivered high knockout rates across a range of cell types, allowing me to investigate gene function in various biological contexts.
United States
11/03/2022
Using TMEM187 Knockout Cell Line-K562 has been a breeze. Its user-friendly workflow and clear instructions have made the gene editing process straightforward, saving me time and effort in the lab.
United States
02/23/2023
TMEM187 Knockout Cell Line-K562 has become an indispensable tool in my research. Its consistent and reliable knockout results have given me the confidence to pursue complex experiments and dive deeper into the mechanisms of gene regulation.
United States
04/11/2023
I highly recommend TMEM187 Knockout Cell Line-K562 to any researcher aiming to explore gene function. Its exceptional performance, combined with its ease of use, has accelerated my research and opened up new avenues of investigation.
United Kingdom
05/09/2023
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