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Cat.No. : CSC-RT0260
Host Cell: 293T Target Gene: TMED9
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0260 |
| Description | 293T-TMED9 (-/-) is a stable cell line with a homozygous knockout of human TMED9 |
| Background | This gene is a member of a family of genes encoding transport proteins located in the endoplasmic reticulum and the Golgi. A similar gene in mouse is the target of microRNA miR-296, which is part of an imprinted cluster. [provided by RefSeq, Jul 2016] |
| Target Gene | TMED9 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
By elucidating the genetic mechanisms behind complex diseases, TMED9 Knockout Cell Line-293T has the potential to revolutionize personalized medicine, leading to more tailored and effective treatment strategies.
United States
01/27/2022
In the realm of drug development, TMED9 Knockout Cell Line-293T has become an invaluable tool for studying the effects of specific genes on drug targets, accelerating the screening process and potentially speeding up the discovery of new therapeutics.
United States
07/19/2022
As I collaborate with colleagues from diverse fields, I have witnessed the cross-disciplinary impact of TMED9 Knockout Cell Line-293T, reinforcing its role as a bridge between genetics and various scientific domains.
United States
11/03/2022
The data generated using TMED9 Knockout Cell Line-293T have not only contributed to peer-reviewed publications but have also attracted interest from industry partners, indicating its potential for commercial applications and furthering scientific innovation.
United Kingdom
02/23/2023
With the continuous advancements in gene editing technology, TMED9 Knockout Cell Line-293T continues to be a driving force in advancing our understanding of genetics, with exciting prospects for its integration into various real-world applications that benefit society as a whole.
United States
04/11/2023
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