Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0255
Target Gene : SURF4 Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0255 |
| Description | 293T-SURF4 (-/-) is a stable cell line with a homozygous knockout of human SURF4 |
| Background | This gene is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein that interacts with endoplasmic reticulum-Golgi intermediate compartment proteins. Disruption of this gene results in reduced numbers of endoplasmic reticulum-Golgi intermediate compartment clusters and redistribution of coat protein I to the cytosol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Target Gene | SURF4 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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SURF4 Knockout Cell Line-293T has truly revolutionized my research, providing unparalleled precision and efficiency in gene editing, and enabling groundbreaking discoveries in gene function.
The easy to do protocol and clear instructions make using SURF4 Knockout Cell Line-293T a breeze, saving time and eliminating unnecessary complexities in the experimental process.
The consistent and reproducible results obtained with SURF4 Knockout Cell Line-293T have bolstered the validity and reliability of my research findings, reinforcing their significance in the scientific community.
The exceptional support provided by the manufacturer, with their prompt responsiveness and insightful guidance, has been instrumental in ensuring the success of my gene knockout experiments.
SURF4 Knockout Cell Line-293T is a game-changer, empowering researchers to delve deeper into the intricacies of genetics and opening up new avenues for scientific exploration and discovery.
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