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SRSF5 Knockout Cell Line-293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0212

Target Gene :   SRSF5 Host Cell :   293T

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0212
Description 293T-SRSF5 (-/-) is a stable cell line with a homozygous knockout of human SRSF5
Target Gene SRSF5
Host Cell 293T
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Background

Q & A

Customer Reviews

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Amazed

I am truly amazed by the transformative impact of SRSF5 Knockout Cell Line-293T on my research, exceeding all expectations and paving the way for groundbreaking discoveries.

United States

Solid

SRSF5 Knockout Cell Line-293T's consistent and reliable performance has provided a solid foundation for my scientific investigations, ensuring accurate and reproducible results that withstand rigorous scrutiny.

United States

Detailed

The friendly interface and detailed instructions have streamlined my experimental workflow, simplifying complex gene knockout procedures and saving valuable time and resources.

United States

Instrumental

The exceptional customer support team has been instrumental, promptly addressing any concerns and providing expert guidance, ensuring a smooth research experience.

United States

Accelerate

I enthusiastically recommend SRSF5 Knockout Cell Line-293T to fellow researchers, as its exceptional efficacy and intuitive design greatly enhance experimental outcomes and accelerate scientific progress.

United States

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