Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0225
Target Gene : SNX3 Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0225 |
| Description | 293T-SNX3 (-/-) is a stable cell line with a homozygous knockout of human SNX3 |
| Background | This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like most family members. This protein interacts with phosphatidylinositol-3-phosphate, and is involved in protein trafficking. A pseudogene of this gene is present on the sex chromosomes. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] |
| Target Gene | SNX3 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
As I embark on future research endeavors, I am confident that SNX3 Knockout Cell Line-293T will remain an integral part of my scientific toolkit, supporting my pursuit of innovative discoveries.
The profound impact of SNX3 Knockout Cell Line-293T goes beyond the confines of my laboratory, as its contributions to the field of genetics reverberate throughout the scientific community.
I am excited to witness the continued advancements in gene editing technology, and I am grateful to have experienced firsthand the transformative power of SNX3 Knockout Cell Line-293T.
As a scientist, I am eager to contribute to the ever-expanding body of knowledge in genetics, and I attribute much of my success to the reliability and efficacy of this remarkable SNX3 Knockout Cell Line-293T.
In the journey of scientific exploration, SNX3 Knockout Cell Line-293T has been a guiding light, illuminating new pathways and enriching my understanding of the intricate genetic landscape.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.