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Cat.No. : CSC-RT0122
Host Cell: SMMC-7721 Target Gene: SIVA1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0122 |
| Description | SMMC-7721-SIVA1 cell line is a stable cell line with a homozygous knockout of SIVA1 |
| Target Gene | SIVA1 |
| Host Cell | SMMC-7721 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The superior quality of SIVA1 Knockout Cell Line-SMMC-7721 has elevated the overall standard of my genetic research, leading to impactful discoveries.
United States
01/27/2022
I appreciate the thorough documentation provided with SIVA1 Knockout Cell Line-SMMC-7721, which helped me navigate through the experimental process smoothly.
United States
07/19/2022
SIVA1 Knockout Cell Line-SMMC-7721 offers excellent value for money, delivering outstanding results without straining my research budget.
United States
11/03/2022
The outstanding specificity of SIVA1 Knockout Cell Line-SMMC-7721 ensures precise targeting of the intended gene, minimizing unwanted off-target effects.
United Kingdom
02/23/2023
I am grateful for the reliability and consistency of SIVA1 Knockout Cell Line-SMMC-7721, as it has become an essential tool in my pursuit of scientific breakthroughs.
United States
04/11/2023
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