Cat.No. : CSC-RT0165
Host Cell: 293T Target Gene: SEPHS1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0165 |
| Description | 293T-SEPHS1 (-/-) is a stable cell line with a homozygous knockout of human SEPHS1 |
| Background | This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010] |
| Target Gene | SEPHS1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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SEPHS1 Knockout Cell Line-293T has exceeded my expectations, delivering exceptional results in my genetic studies and providing a powerful tool for precise gene manipulation.
I am completely impressed by the high efficiency and accuracy of SEPHS1 Knockout Cell Line-293T, enabling me to achieve targeted gene modifications with remarkable success rates.
The user-friendly nature of SEPHS1 Knockout Cell Line-293T's protocol has made it straightforward to incorporate into my experimental workflow, saving time and ensuring consistent results.
The reproducibility and reliability of the outcomes obtained using SEPHS1 Knockout Cell Line-293T have reinforced the robustness of my research findings, increasing their impact and significance.
I appreciate the outstanding technical support provided by the manufacturer, who has been responsive and knowledgeable, offering valuable assistance throughout my gene knockout experiments.
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