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Cat.No. : CSC-RT0281
Host Cell: 293T Target Gene: SEC11A
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0281 |
| Description | 293T-SEC11A (-/-) is a stable cell line with a homozygous knockout of human SEC11A |
| Background | This gene encodes a member of the peptidase S26B family. The encoded protein is an 18kDa subunit of the signal peptidase complex and has been linked to cell migration and invasion, gastric cancer and lymph node metastasis. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, Dec 2012] |
| Target Gene | SEC11A |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The confidence instilled by SEC11A Knockout Cell Line-293T has encouraged me to tackle ambitious research projects, pushing the boundaries of my scientific curiosity and embracing the thrill of exploration.
United States
07/19/2022
Beyond its technical capabilities, SEC11A Knockout Cell Line-293T has fostered a sense of camaraderie among fellow researchers, as we share insights and collaborate on projects united by our common reliance on this invaluable tool.
United States
11/03/2022
With each milestone achieved using SEC11A Knockout Cell Line-293T, I am reminded of the profound impact that science and technology can have on addressing complex challenges and shaping a better world for future generations.
United States
02/23/2023
As I navigate the twists and turns of my research journey, I remain grateful for the support and possibilities that SEC11A Knockout Cell Line-293T has offered, serving as a beacon of progress in the ever-evolving landscape of genetics.
United States
04/11/2023
With determination in my heart and this exceptional gene knockout reagent in my hand, I look forward to the chapters that lie ahead, eager to contribute to the ongoing exploration of life's genetic blueprint and to leave my mark in the fascinating saga of scientific discovery.
United States
08/23/2020
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