Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0231
Target Gene : SARNP Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0231 |
| Description | 293T-SARNP (-/-) is a stable cell line with a homozygous knockout of human SARNP |
| Background | This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting in expression of a chimeric protein, has been associated with acute myelomonocytic leukemia. Pseudogenes exist on chromosomes 7 and 8. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009] |
| Target Gene | SARNP |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
In the realm of genetic research, SARNP Knockout Cell Line-293T stands as a reliable and indispensable tool, supporting us in our quest to decipher the intricate mechanisms of life.
With its precise and efficient gene editing capabilities, SARNP Knockout Cell Line-293T empowers us to unravel the complexities of gene function, paving the way for breakthrough discoveries.
The straightforward protocol and user-friendly nature of SARNP Knockout Cell Line-293T make it accessible to us of various backgrounds, fostering collaboration and knowledge-sharing within the scientific community.
Through the consistent and reproducible results it yields, SARNP Knockout Cell Line-293T instills confidence in researchers, providing a solid foundation for robust scientific conclusions and further investigation.
The support and expertise offered by the manufacturer have been instrumental in ensuring the successful application of SARNP Knockout Cell Line-293T, enhancing the overall research experience and driving scientific progress.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.