Robust Tn5 Transposase

Name: Robust Tn5 Transposase
SKU: EMQZ1422
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1422

Inquire for Price

Product Information

Cat. No.	EMQZ1422
Description	Robust Tn5 Transposase is a hyperactive form of Tn5 transposase. This enzyme can be used to randomly insert Tn5 transposon into target DNA. Robust Tn5 Transposase recognizes inside end sequences, outside end sequences and mosaic end sequences of Tn5 transposon.
Source	An E.coli strain that carries Robust Tn5 transposase gene.
Concentration	1,000 U/ml
Applications	• in vitro transgenic experiment • Construction of random library for second-generation sequencing
Size	25 U
Unit Definition	One unit Tn5 transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hourat 37°C.
Storage	Store at -20°C

Quick Inquiry

Publications

Q & A

Customer Reviews

Publications

Man N, Mas G, Karl D L, et al. p300 suppresses the transition of myelodysplastic syndromes to acute myeloid leukemia[J]. JCI insight, 2021, 6(19).
Leong P P T, Mihajlović A, Bogdanović N, et al. Reconstructing and counting genomic fragments through tagmentation-based haploid phasing[J]. Scientific Reports, 2021, 11(1): 18907.
Gould G M, Grauman P V, Theilmann M R, et al. Detecting clinically actionable variants in the 3′ exons of PMS2 via a reflex workflow based on equivalent hybrid capture of the gene and its pseudogene[J]. BMC Medical Genetics, 2018, 19: 1-13.
Day R C, Godwin T D, Harris C, et al. Integrated cell expression toolkit for low input transcript profiling[J]. bioRxiv, 2018: 458851.

Customer Q&As

What experiments can this reagent be used for?

A: This transposase can be used for in vitro transgenic experiments and construction of second-generation sequencing random libraries.

Is the reaction buffer included in the reagent?

A: Yes, the product contains enzyme, 5× lm buffer, and 10× tps buffer.

What is the relationship between Robust Tn5 Transposase and Tn5 Transposase?

A: Robust Tn5 Transposase is a hyperactive form of Tn5 transposase.

Can you tell me when the reagents usually expire?

A: it's usually about 2 years, and for specific lots the expiration is indicated on the product label.

What is the concentration of your Robust Tn5 Transposase?

A: The concentration of Tn5 transposase is 1.8-3.7uM/L (equal to 1.8-3.7nM/mL and 0.1-0.2 mg/mL).

Is your Robust Tn5 Transposase preloaded with adaptor or not?

A: No. Our products only contain Robust Tn5 Transposase and reaction buffers. They do not come preloaded with adaptors.

What conditions would be recommended for enzyme deactivation?

A: 0.1% SDS or heat (> 68°C) for 5-10 min.

How many experiments could I envisage to do with the 25U?

A: It is difficult to estimate the dosage. Starting with 1U per experiment is recommended, but the exact dosage depends on various factors such as the length of the fragment, the efficiency of the competent cell, and the specific cell type used.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

High recognition accuracy

The Robust Tn5 Transposase recognizes the internal end sequences, external end sequences, and junction end sequences of the Tn5 transposon.

United States

2022-08-05

Random insertion

This enzyme randomly inserts the Tn5 transposon into the target DNA.

United States

2023-03-15

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Robust Tn5 Transposase

Product Information

Publications

Q & A

Customer Reviews

Publications

What experiments can this reagent be used for?

Is the reaction buffer included in the reagent?

What is the relationship between Robust Tn5 Transposase and Tn5 Transposase?

Can you tell me when the reagents usually expire?

What is the concentration of your Robust Tn5 Transposase?

Is your Robust Tn5 Transposase preloaded with adaptor or not?

What conditions would be recommended for enzyme deactivation?

How many experiments could I envisage to do with the 25U?

High recognition accuracy

Random insertion

Related Products

Related Services