Cat.No. : CSC-RT0104
Host Cell: NIH/3T3 Target Gene: Rock1Rock2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0104 |
| Description | NIH-3T3-ROCK1/ROCK2 cell line is a stable cell line with a double homozygous knockout of ROCK1 and ROCK2 |
| Target Gene | Rock1Rock2 |
| Host Cell | NIH/3T3 |
| Host Cell Species | Mus musculus (Mouse) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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ROCK1/ROCK2 Knockout Cell Line-NIH-3T3 has truly been a highly competent assistants in my research. Its exceptional knockout efficiency and reliable performance have allowed me to uncover the functional importance of specific genes with remarkable precision.
I am deeply impressed with the effectiveness of ROCK1/ROCK2 Knockout Cell Line-NIH-3T3. Its consistent and reproducible knockout results have provided me with a solid foundation for understanding gene function and its impact on biological processes.
Using ROCK1/ROCK2 Knockout Cell Line-NIH-3T3 has been a seamless experience. Its user-friendly interface and efficient knockout capabilities have simplified the gene editing process and saved me valuable time and effort in the lab.
I can't say it enough the value of ROCK1/ROCK2 Knockout Cell Line-NIH-3T3 in my research endeavors. Its high knockout efficiency and consistent results have enabled me to explore the intricate mechanisms of gene regulation and make significant scientific breakthroughs.
I recommend ROCK1/ROCK2 Knockout Cell Line-NIH-3T3 to fellow researchers. Its exceptional performance, ease of use, and reliable knockout outcomes have elevated the quality and impact of my experiments, leading to valuable insights and discoveries.
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