RNA 5′ Polyphosphatase

Name: RNA 5′ Polyphosphatase
SKU: EMPT3004
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMPT3004

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Product Information

Cat. No.	EMPT3004
Description	RNA 5′ Polyphosphatase* is a Mg²⁺-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the γ and β phosphates from 5′-triphosphorylated RNA (such as primary RNA transcripts):5′ pppN-OH 3′ → 5′ pN-OH 3′ + 2 PiRNAs with a 5′-diphosphorylated end are also converted to 5′-monophosphorylated RNA by RNA 5′ Polyphosphatase:5′ ppN-OH 3′ → 5′ pN-OH 3′ + PiRNA Polyphosphatase has no activity on RNA with a 5′ cap (e.g., 5′ m7GpppN-OH 3′), or a 5′-monophosphorylated end (5′ pN-OH 3′). However, both NTPs and dNTPs are substrates for the enzyme, yielding the corresponding NMPs and dNMPs + inorganic phosphate: (d)NTP → (d)NMP + 2Pi
Concentration	20000 units/ml
Applications	Conversion of 5′-triphosphorylated RNA to 5′-monophosphorylated RNA for use in 5′-RNA ligation-tagging methods using T4 RNA Ligase. Analysis of 5′-end structure of RNA. Preparation of substrate RNA molecules for subsequent degradation using Epicentre"s Terminator? 5′-Phosphate-Dependent Exonuclease.
Size	200 units
Unit Definition	One unit of RNA 5′ Polyphosphatase releases 1 nmol of inorganic phosphate from ATP in 1 hour at 37 °C under standard assay conditions.
Storage	50% glycerol containing 0.05 M Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.

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What is the specificity of the RNA 5′ Polyphosphatase?

A: The RNA 5′ Polyphosphatase removes γ and β phosphates from 5'-triphosphorylated RNA and β phosphates from diphosphorylated RNA, but it does not dephosphorylate monophosphorylated or 5'-capped RNA.

How is the RNA 5′ Polyphosphatase applied?

A: The RNA 5′ Polyphosphatase is applicable for 5´-RNA labeling using T4 RNA Ligase, analysis of RNA 5´ end structure, and preparation of substrate RNA for degradation with Terminator 5´-Phosphate-Dependent Exonuclease.

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