Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : QBHC-01
| Cat. No. | QBHC-01 |
| Description |
Premix qRT Master Mix Kit with ROX is a specifically designed for fast and sensitive real-time PCR via either SYBR Green Real-Time PCR (qPCR) or Taqman Real-Time PCR (qPCR) requiring normalization with ROX. SYBR Green I Dye and TaqMan probes are not included in this RT-PCR kit. The Premix qRT Master Mix Kit with ROX consists of high fidelity and high performance Hot Start polymerase and a newly formulated, real-time buffer that ensures superior specificity and increased amplification efficiency during RT-PCR (qPCR). |
| Sample Type | RNA |
| Report Gene | Both SYBR Green and probe and single tube reaction |
| Features |
•High specificity and increased amplification efficiency for qPCR •Sensitivity for SYBR Green I or probe detection •Compatibility with either TaqMan Probes or SYBR Green I Dye •Compatibility with many commonly used Real-Time PCR instruments |
| Applications | Real-Time PCR using either Taqman Probes or SYBR Green I Dye |
| Selection Marker | RNA |
| Sensitivity | Variable |
| Method | Probe and SYBR Green I Dye based |
| Note | The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option. |
| Storage | Store at -20°C and at dark condtion |
A: ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix.
A: Unlike SYBR Green in qPCR, ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.
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