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Premix qRT Master Mix Kit (with ROX)

For research use only. Not intended for any clinical use.
Cat.No.
QBHC-01
Description
Premix qRT Master Mix Kit with ROX is a specifically designed for fast and sensitive real-time PCR via either SYBR Green Real-Time PCR (qPCR) or Taqman Real-Time PCR (qPCR) requiring normalization with ROX. SYBR Green I Dye and TaqMan probes are not included in this RT-PCR kit. The Premix qRT Master Mix Kit with ROX consists of high fidelity and high performance Hot Start polymerase and a newly formulated, real-time buffer that ensures superior specificity and increased amplification efficiency during RT-PCR (qPCR).

Applications
Real-Time PCR using either Taqman Probes or SYBR Green I Dye
Features
•High specificity and increased amplification efficiency for qPCR
•Sensitivity for SYBR Green I or probe detection
•Compatibility with either TaqMan Probes or SYBR Green I Dye
•Compatibility with many commonly used Real-Time PCR instruments
Method
Probe and SYBR Green I Dye based
Note
The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Report Gene
Both SYBR Green and probe and single tube reaction
Sample Type
RNA
Selection Marker
RNA
Sensitivity
Variable
Storage
Store at -20°C and at dark condtion

Publications

Q & A

Customer Reviews

Publications
  1. Huang Z, Li Y, Luo Y, et al. Human milk oligosaccharides 3′-sialyllactose and 6′-sialyllactose protect intestine against necrotizing enterocolitis damage induced by hypoxia[J]. Journal of Functional Foods, 2021, 86: 104708.
Customer Q&As
What is ROX?

A: ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix.

What is the difference between ROX and SYBR Green?

A: Unlike SYBR Green in qPCR, ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.

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