PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble - just add RNA template and water. This kit is designed for two-step real-time RT-PCR. Use it upstream of a SYBR or TaqMan qPCR premix.
This kit includes Reverse Transcriptase, which has exceptionally strong strand displacement activity for optimal efficiency during cDNA synthesis.
•Reverse transcription in just 15 minutes
•Master Mix format reduces pipetting steps and the possibility of contamination
•Works efficiently with GC-rich and/or higher-structured RNA templates
•Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis
Real-Time PCR using either Taqman Probes or SYBR Green I Dye
Probe and SYBR Green I Dye based
Two-step real-time RT-PCR
Store at -20°C and at dark condtion
For Research Use Only. Not for use in diagnostic procedures.