Premix qRT Master Mix Kit

Name: Premix qRT Master Mix Kit
SKU: QBHC-02
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : QBHC-02

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Product Information

Cat. No.	QBHC-02
Description	PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble - just add RNA template and water. This kit is designed for two-step real-time RT-PCR. Use it upstream of a SYBR or TaqMan qPCR premix. This kit includes Reverse Transcriptase, which has exceptionally strong strand displacement activity for optimal efficiency during cDNA synthesis.
Sample Type	RNA
Report Gene	Both SYBR Green and probe and two tube reaction
Features	•Reverse transcription in just 15 minutes •Master Mix format reduces pipetting steps and the possibility of contamination •Works efficiently with GC-rich and/or higher-structured RNA templates •Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis
Applications	Real-Time PCR using either Taqman Probes or SYBR Green I Dye
Selection Marker	DNA
Sensitivity	Variable
Method	Probe and SYBR Green I Dye based
Storage	Store at -20°C and at dark condtion

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What is reverse transcriptase?

A: A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template.

What is the thermal stability of reverse transcriptase?

A: The ability of reverse transcriptase to withstand high temperatures is an important factor affecting cDNA synthesis. Elevating the reaction temperature helps denature RNA with strong secondary structure and/or high GC content, allowing reverse transcriptase to read the sequence.

What does reverse transcriptase fidelity mean?

A: The fidelity of reverse transcriptase refers to the sequence accuracy during reverse transcription of RNA into DNA. Fidelity is inversely proportional to reverse transcription error rate.

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