The Plasmid Endo-Free Ultrapure Kit integrates an efficient endotoxin removal step into the plasmid purification procedure to make it the fastest Endo-Free prep method available. Purified, Endo-Free DNA is suitable for transfection and injection. Large numbers of clones that require Endo-Free DNA can be rapidly purified using this kit. No additional affinity columns to remove lipopolysaccharides are required. The plasmid DNA is bound to a silica spin filter, washed once and released into Endo-Free water. The plasmid DNA is concentrated enough to be used directly in transfection experiments. Endotoxins, also known as lipopolysaccharides, are cell membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.
Results in high quality plasmid DNA
Produces DNA suitable for transfection and injection
Utilizes a simple silica spin filter for DNA yields equal to anion exchange columns
Rapid protocol with no additional affinity columns required to remove lipopolysa
Nucleic Acid Type
Alkaline Lysis/Endo removal
High purity, suitable for any downstream applications.
All the kit components are guaranteed for at least 12 months from the date of purchase.
For Research only.