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Plasmid Endo-Free Ultrapure Kit

Cat.No.
NKP-T019
Description
The Plasmid Endo-Free Ultrapure Kit integrates an efficient endotoxin removal step into the plasmid purification procedure to make it the fastest Endo-Free prep method available. Purified, Endo-Free DNA is suitable for transfection and injection. Large numbers of clones that require Endo-Free DNA can be rapidly purified using this kit. No additional affinity columns to remove lipopolysaccharides are required. The plasmid DNA is bound to a silica spin filter, washed once and released into Endo-Free water. The plasmid DNA is concentrated enough to be used directly in transfection experiments. Endotoxins, also known as lipopolysaccharides, are cell membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.
Features
Results in high quality plasmid DNA
Produces DNA suitable for transfection and injection
Utilizes a simple silica spin filter for DNA yields equal to anion exchange columns
Rapid protocol with no additional affinity columns required to remove lipopolysa
Sample Type
Endo-Free Plasmid
Nucleic Acid Type
Plasmid DNA
Technology
Alkaline Lysis/Endo removal
Quality
High purity, suitable for any downstream applications.
Time
10 minutes
Throughput
1-24 samples
Storage conditions
All the kit components are guaranteed for at least 12 months from the date of purchase.
Regulatory Statement
For Research only.

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