Plasmid Endo-Free Ultrapure Kit

Name: Plasmid Endo-Free Ultrapure Kit
SKU: NKP-T019
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : NKP-T019

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Product Information

Cat. No.	NKP-T019
Description	The Plasmid Endo-Free Ultrapure Kit integrates an efficient endotoxin removal step into the plasmid purification procedure to make it the fastest Endo-Free prep method available. Purified, Endo-Free DNA is suitable for transfection and injection. Large numbers of clones that require Endo-Free DNA can be rapidly purified using this kit. No additional affinity columns to remove lipopolysaccharides are required. The plasmid DNA is bound to a silica spin filter, washed once and released into Endo-Free water. The plasmid DNA is concentrated enough to be used directly in transfection experiments. Endotoxins, also known as lipopolysaccharides, are cell membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.
Sample Type	Endo-Free Plasmid
Report Gene	Endo-Free Plasmid
Features	· Results in high quality plasmid DNA · Produces DNA suitable for transfection and injection · Utilizes a simple silica spin filter for DNA yields equal to anion exchange columns · Rapid protocol with no additional affinity columns required to remove lipopolysa
Manipulation	10 minutes
Nucleic Acid Type	Plasmid DNA
Promoter	Alkaline Lysis/Endo removal
Selection	Multiple samples
Selection Marker	Plasmid DNA
Throughput	1-24 samples
Quality	High purity, suitable for any downstream applications.
Regulatory Statement	For Research only.
Storage	All the kit components are guaranteed for at least 12 months from the date of purchase.

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