Panoply™ Human TWIST1 Over-expressing Stable Cell Line

Name: Panoply™ Human TWIST1 Over-expressing Stable Cell Line
SKU: CSC-SC016883
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016883

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC016883
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TWIST1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TWIST1 twist homolog 1 (Drosophila) [ Homo sapiens ]
Gene Symbol	TWIST1
Synonyms	SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Gene Description	twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila)
Gene ID	7291
Uni Prot ID	Q15672
m RNA Refseq	NM_000474.3
Protein Refseq	NP_000465.1
Chromosome Location	7p21.2
Function	E-box binding; bHLH transcription factor binding; protein binding; protein domain specific binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding RNA polymerase II transcription factor activity; transcription factor binding;
Pathway	Adipogenesis, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Neural Crest Differentiation, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem;
MIM	601622

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