Panoply™ Human TRIM28 Over-expressing Stable Cell Line

Name: Panoply™ Human TRIM28 Over-expressing Stable Cell Line
SKU: CSC-SC016580
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016580

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC016580
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TRIM28
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TRIM28 tripartite motif containing 28 [ Homo sapiens ]
Gene Symbol	TRIM28
Synonyms	KAP1; TF1B; RNF96; TIF1B
Gene Description	tripartite motif-containing 28
Gene ID	10155
Uni Prot ID	Q13263
m RNA Refseq	NM_005762.2
Protein Refseq	NP_005753.1
Chromosome Location	19q13.4
Function	DNA binding; chromo shadow domain binding; protein binding; protein kinase activity; contributes_to sequence-specific DNA binding; contributes_to sequence-specific DNA binding transcription factor activity; transcription coactivator activity; transcription corepressor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding;
Pathway	C-MYB transcription factor network, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; p53 pathway, organism-specific biosystem;
MIM	601742

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