Panoply™ Human TNKS2 Over-expressing Stable Cell Line

Name: Panoply™ Human TNKS2 Over-expressing Stable Cell Line
SKU: CSC-SC016391
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016391

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC016391
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TNKS2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNKS2 tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [ Homo sapiens ]
Gene Symbol	TNKS2
Synonyms	TNKL; ARTD6; TANK2; pART6; PARP5B; PARP5C; PARP-5b; PARP-5c
Gene Description	tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
GeneID	80351
Uni ProtID	Q9H2K2
mRNA Refseq	NM_025235.3
Protein Refseq	NP_079511.1
Chromosome Location	10q23.3
Function	NAD+ ADP-ribosyltransferase activity; enzyme binding; metal ion binding; protein binding;
MIM	607128

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Tankyrase 2 (TNKS2) is a potential molecular target for the prognosis and treatment of non-small cell lung cancer (NSCLC), but its biological function remains unclear. Here, researchers show that TNKS2 mRNA and protein expression are significantly increased in highly malignant NCI-H647 cells, while remaining at lower levels in low-malignant A549 cells. Compared to the control group, the TNKS2 overexpression group showed a significantly reduced apoptosis rate (50%) and a significantly increased cell migration rate (35%). In contrast, shTNKS2 increased apoptosis by more than 1-fold and reduced cell migration rate by 60%. Immunofluorescence analysis showed enhanced localization of β-catenin fluorescence signal in the cell nucleus, which was correlated with high TNKS2 expression levels. Western blot analysis of TNKS2/β-catenin-related proteins showed that the expression changes of TNKS2 and β-catenin in lung cancer cells were consistent, while the expression changes of Axin showed the opposite trend. These results suggest that TNKS2 may serve as a poor prognostic factor and a potential therapeutic target for non-small cell lung cancer (NSCLC).

Here, researchers evaluated the proliferation of non-small cell lung cancer (NSCLC) cells with TNKS2 knockdown and TNKS2 overexpression. Compared to the control group, H647 cells showed the same proliferation rate as TNKS2-silenced cells, while TNKS2-overexpressing A549 cells showed the same proliferation rate as non-overexpressing A549 cells (Figure 1A). Flow cytometry was used to analyze the effects of TNKS2 knockdown or overexpression on NSCLC cell apoptosis. The results showed that TNKS2 knockdown significantly increased the apoptosis rate of H647 cells compared to the control group. Conversely, TNKS2 overexpression significantly inhibited apoptosis in A549 cells compared to the control group (Figure 1B). These results indicate that TNKS2 expression is negatively correlated with apoptosis in non-small cell lung cancer (NSCLC) cells. Furthermore, TNKS2 knockdown significantly reduced the scratch healing ability of H647 cells, suggesting that TNKS2 knockdown weakens cell migration. Conversely, TNKS2 overexpressing A549 cells showed significantly enhanced scratch healing ability, indicating that TNKS2 overexpression enhances the migration ability of A549 cells (Figure 1C).

Figure 1. Effects of tankyrase 2 knockdown or overexpression on proliferation of non-small cell lung cancer cells. (Wang Y, Zhang Y J., 2024)

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