Panoply™ Human TNFSF10 Over-expressing Stable Cell Line

Name: Panoply™ Human TNFSF10 Over-expressing Stable Cell Line
SKU: CSC-SC016371
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016371

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC016371
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TNFSF10
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 [ Homo sapiens ]
Gene Symbol	TNFSF10
Synonyms	TNFSF10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10; Apo 2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; APO2L; Apo-2L;
GeneID	8743
Uni ProtID	P50591
mRNA Refseq	BC032722
Chromosome Location	3q26
Function	cytokine activity; metal ion binding; protein binding; receptor binding; tumor necrosis factor receptor binding;
Pathway	Activation of Pro-Caspase 8, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; Caspase-8 is formed from procaspase-8, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem;
MIM	603598

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Mesenchymal stem cell-based tumor therapy is currently limited by insufficient secretion of effector factors and discrepancies between in vitro and in vivo therapeutic efficacy. Here, researchers investigated whether genetically engineered adipose-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL, also known as TNFSF10) could inhibit H460 tumor growth. They generated genetically engineered ASCs overexpressing TRAIL and interferon-beta (IFN-β), designated as ASC-TRAIL and ASC-IFN-β, respectively. Through apoptosis and cytotoxicity assays, the researchers evaluated the death of H460 cells co-cultured with-or exposed to the conditioned media of-ASCs, ASC-TRAIL, and ASC-IFN-β. The results demonstrated that conditioned medium from ASC-IFN-β induced H460 cell apoptosis more significantly than that from ASC-TRAIL. In an H460 xenograft model, while unmodified ASCs promoted tumor growth, both ASC-TRAIL and ASC-IFN-β significantly inhibited it. Notably, in the ASC-IFN-β group, tumors developed in only 20% of the nude mice, and the resulting tumors were smaller in both volume and weight compared to the control group. In conclusion, TRAIL-overexpressing ASCs hold potential for tumor therapy; among them, ASC-IFN-β cells are capable of secreting higher levels of TRAIL.

To determine whether TRAIL-overexpressing ASCs exhibited cytotoxicity against H460 lung cancer cells, the researchers treated H460 cells with conditioned medium (CM) collected after 2 days of culturing ASC-TRAIL and ASC-IFN-β cells. The results showed that CM from ASC-TRAIL and ASC-IFN-β induced cytotoxicity levels of approximately 42.65% ± 0.04% and 55.76% ± 0.05%, respectively, in H460 cells (Figure 1A). Although H460 cells exhibited dose-dependent sensitivity to exogenous TRAIL, treatment with 1,000 U/ml of IFN-β resulted in only about 13.3% cytotoxicity (Figure 1B). Therefore, the cytotoxic effects induced by ASC-TRAIL and ASC-IFN-β CM in H460 cells were TRAIL-dependent.

Figure 1. Cytotoxicity of H460 cells induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and interferon (IFN)-β. (UN CHOI Y, et al., 2021)

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