Panoply™ Human TBK1 Knockdown Stable Cell Line

Name: Panoply™ Human TBK1 Knockdown Stable Cell Line
SKU: CSC-DC015668
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC015668

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

Inquire for Price

Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC015668
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	TBK1
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TBK1 TANK-binding kinase 1 [ Homo sapiens ]
Gene Symbol	TBK1
Synonyms	TBK1; TANK-binding kinase 1; serine/threonine-protein kinase TBK1; NAK; NF-kB-activating kinase; NF-kappa-B-activating kinase; T2K; FLJ11330;
GeneID	29110
Uni ProtID	Q9UHD2
mRNA Refseq	BC034950
Chromosome Location	12q14.2
Function	ATP binding; nucleic acid binding; nucleotide binding; phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity;
Pathway	Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Hepatitis C, organism-specific biosystem;
MIM	604834

Quick Inquiry

Case Study

Q & A

Customer Reviews

Cholangiocarcinoma (CCA) is a highly heterogeneous and metastatic malignant tumor with a poor prognosis even after radical hepatectomy. Here, researchers found that serine/threonine protein kinase TANK-binding kinase 1 (TBK1) exhibits dynamic changes at different stages of spontaneous CCA carcinogenesis in mice (hyperplasia, dysplasia, and CCA). Compared to non-tumor tissues, TBK1 is highly expressed in human tissues, including intrahepatic (n=182) and extrahepatic (n=40) CCA tissues, and elevated TBK1 expression is positively correlated with tumor diameter increase, lymph node metastasis, and TNM stage progression. Functional studies showed that TBK1 promotes CCA growth and metastasis both in vitro and in vivo. TBK1 directly interacts with β-catenin, promoting its phosphorylation at the S552 site and its nuclear translocation, thereby activating EMT-related transcriptional reprogramming. The TBK1 inhibitor GSK-8612 or a kinase-inactivating mutant effectively inhibited this process.

To elucidate the role of TBK1 in the progression of cholangiocarcinoma (CCA), researchers knocked down TBK1 expression in HuCCT1 cells, which have relatively high levels of endogenous TBK1. Furthermore, they overexpressed TBK1 in TFK1 cells, which have relatively low levels of endogenous TBK1. CCK8 assays showed that cell proliferation was inhibited in TBK1-knockdown cells. Scratch-migration, Transwell migration, and Matrigel invasion assays demonstrated that the growth, migration, and invasion abilities of TBK1-knockdown cells were weakened (Figures 1A and 1B). Conversely, TBK1 overexpression significantly enhanced these effects.

Figure 1. TBK1 promotes CCA cell growth, migration, and invasion. (Gao C Q, et al., 2023)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *