Panoply™ Human SPI1 Over-expressing Stable Cell Line

Name: Panoply™ Human SPI1 Over-expressing Stable Cell Line
SKU: CSC-SC015044
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC015044

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC015044
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	SPI1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1 [ Homo sapiens ]
Gene Symbol	SPI1
Synonyms	OF; PU.1; SFPI1; SPI-1; SPI-A
Gene Description	spleen focus forming virus (SFFV) proviral integration oncogene spi1
GeneID	6688
Uni ProtID	P17947
mRNA Refseq	NM_001080547.1
Protein Refseq	NP_001074016.1
Chromosome Location	11p11.2
Function	NFAT protein binding; RNA binding; RNA polymerase II distal enhancer sequence-specific DNA binding; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding; core promoter binding; protein binding; sequence-specific DNA binding transcription factor activity;
Pathway	Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Epstein-Barr virus infection, organism-specific biosystem; Epstein-Barr virus infection, conserved biosystem; Glucocorticoid receptor regulatory network, organism-specific biosystem; HTLV-I infection, organism-specific biosystem;
MIM	165170

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