Panoply™ Human RIT1 Over-expressing Stable Cell Line

Name: Panoply™ Human RIT1 Over-expressing Stable Cell Line
SKU: CSC-SC013328
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC013328

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC013328
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	RIT1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	RIT1 Ras-like without CAAX 1 [ Homo sapiens ]
Gene Symbol	RIT1
Synonyms	RIT1; Ras-like without CAAX 1; Ric (Drosophila) like, expressed in many tissues , RIT; GTP-binding protein Rit1; GTP binding protein Roc1; MGC125864; MGC125865; RIBB; Ric like; expressed in many tissues; ROC1; GTP-binding protein Roc1; ras-like without CAAX protein 1; Ric-like, expressed in many tissues; ras-like protein expressed in many tissues; RIT;
Gene ID	6016
Uni Prot ID	Q92963
m RNA Refseq	BC104186
Chromosome Location	1q21.2
Function	GTP binding; GTPase activity; calmodulin binding; nucleotide binding; protein binding;
Pathway	NGF signalling via TRKA from the plasma membrane, organism-specific biosystem; Neurotrophic factor-mediated Trk receptor signaling, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signalling by NGF, organism-specific biosystem; Signalling to ERKs, organism-specific biosystem; Signalling to p38 via RIT and RIN, organism-specific biosystem; Trk receptor signaling mediated by the MAPK pathway, organism-specific biosystem;
MIM	609591

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