Panoply™ Human RAF1 Over-expressing Stable Cell Line

Name: Panoply™ Human RAF1 Over-expressing Stable Cell Line
SKU: CSC-SC012928
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC012928

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC012928
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	RAF1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	RAF1 v-raf-1 murine leukemia viral oncogene homolog 1 [ Homo sapiens ]
Gene Symbol	RAF1
Synonyms	RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; RAF proto-oncogene serine/threonine-protein kinase; c Raf; CRAF; Raf 1; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; NS5; Raf-1; c-Raf;
GeneID	5894
Uni ProtID	P04049
mRNA Refseq	BC018119
Chromosome Location	3p25
Function	ATP binding; MAP kinase kinase kinase activity; Ras GTPase binding; metal ion binding; mitogen-activated protein kinase kinase binding; nucleotide binding; protein binding; protein heterodimerization activity; protein kinase acti
Pathway	ARMS-mediated activation, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem;
MIM	164760

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Raf-1 proto-oncogene, serine/threonine kinase (Raf1), is a crucial component of the RAS/RAF/MEK/ERK signaling pathway, regulating cell migration, apoptosis, and differentiation. However, research on Raf1 expression and its clinical significance in non-small cell lung cancer (NSCLC) is limited. Here, researchers explored the clinical value and prognostic significance of Raf1 in NSCLC patients after radiotherapy. The study found that Raf1 expression was significantly associated with the invasive and metastatic abilities of lung cancer A549 and H1299 cell lines. Results showed that Raf1 expression was present in 44.5% of NSCLC patient specimens, and Raf1 expression was positively correlated with lymph node metastasis, T stage, and histological poor differentiation. Patients with negative or low Raf1 expression experienced slower disease progression than those with high Raf1 expression. Multivariate analysis indicated that Raf1 was an independent prognostic factor for time to progression (TTP). High Raf1 expression was associated with poorer 3-year overall survival (OS). Raf1 overexpression was associated with early progression of NSCLC. Raf1 may become a novel prognostic factor and potential target for improving the long-term prognosis of NSCLC patients.

Here, researchers performed in vitro gain-of-function analyses to investigate the effects of Raf1 on lung cancer cell invasion and migration. Scratch healing assays showed that Raf1-overexpressing A549 cells exhibited enhanced lateral migration, while Raf1-silenced H1299 cells showed diminished lateral migration (Figure 1). Transwell invasion assays also demonstrated that Raf1-overexpressing A549 cells exhibited enhanced invasiveness, while Raf1-silenced H1299 cells showed diminished invasiveness (Figure 2). In summary, these results indicate that Raf1 can promote the migration and invasion of lung cancer cells.

Figure 1. Wound healing assay showed that Raf1 expression affects the lateral migration in lung cancer cells. (Tian H, et al., 2018)

Figure 2. Transwell invasion assay shows that the Raf1 expression affects the invasion ability in lung cancer cells. (Tian H, et al., 2018)

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