Panoply™ Human PTK2 Over-expressing Stable Cell Line

Name: Panoply™ Human PTK2 Over-expressing Stable Cell Line
SKU: CSC-SC012678
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC012678

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC012678
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PTK2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PTK2 PTK2 protein tyrosine kinase 2 [ Homo sapiens ]
Gene Symbol	PTK2
Synonyms	FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Gene Description	PTK2 protein tyrosine kinase 2
GeneID	5747
Uni ProtID	Q05397
mRNA Refseq	NM_001199649.1
Protein Refseq	NP_001186578.1
Chromosome Location	8q24.3
Pathway	Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Angiogenesis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic execution phase, organism-specific biosystem;
MIM	600758

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Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. More than half of UM cases develop distant metastases, including to the liver, lungs, bones, and other organs, which often leads to patient death. However, the mechanisms of UM metastasis remain largely unknown. Here, researchers found that non-receptor protein tyrosine kinase 2 (PTK2, also known as focal adhesion kinase, FAK) is overexpressed in most UM samples tested. Furthermore, they found that PTK2 expression is a novel independent risk factor that can predict poor prognosis in UM patients. Mechanistic studies showed that PTK2 promotes an epithelial-mesenchymal transition (EMT) phenotype, leading to UM cell metastasis. PTK2, located on chromosome 8q, is a functional gene whose gain on chromosome 8q contributes to UM metastasis, potentially representing a molecular mechanism for the abnormal expression and activation of PTK2 in UM. These data reveal a novel role and mechanism of PTK2 in UM metastasis, suggesting that PTK2 could be a potential prognostic biomarker for UM metastasis and a promising therapeutic target for UM treatment.

Uveal melanoma (UM) metastasis is a major cause of treatment failure and often leads to patient death. To investigate whether PTK2 affects UM cell metastasis, researchers studied the effect of PTK2 on metastasis by upregulating or inhibiting PTK2 expression in UM cells. Wound healing and Transwell invasion assays showed that PTK2-overexpressing UM cells exhibited enhanced migration and invasion capabilities (Figure 1A-C). The opposite effect was observed in cells with PTK2 inactivation (Figure 1A-C). The researchers confirmed that PTK2 mRNA levels were significantly increased and decreased in PTK2-overexpressing and PTK2-knockdown UM cells, respectively (Figure 1D). They also assessed PTK2 activation using immunofluorescence. Compared to control cells, PTK2-overexpressing cells showed higher levels of phosphorylated PTK2 expression, while PTK2-silenced cells and cells treated with a PTK2 inhibitor showed lower levels of phosphorylated PTK2 expression (Figure 1E).

Figure 1. Protein tyrosine kinase 2 (PTK2) enhances the metastasis of uveal melanoma in vitro. (Luo P, et al., 2023)

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