Panoply™ Human PRMT5 Over-expressing Stable Cell Line

Name: Panoply™ Human PRMT5 Over-expressing Stable Cell Line
SKU: CSC-SC012428
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC012428

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC012428
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PRMT5
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PRMT5 protein arginine methyltransferase 5 [ Homo sapiens ]
Gene Symbol	PRMT5
Synonyms	JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
Gene Description	protein arginine methyltransferase 5
Gene ID	10419
Uni Prot ID	O14744
m RNA Refseq	NM_001039619.1
Protein Refseq	NP_001034708.1
Chromosome Location	14q11.2
Pathway	E2F transcription factor network, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolism of non-coding RNA, organism-specific biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; p53 pathway, organism-specific biosystem;
MIM	604045

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