Panoply™ Human PPM1K Over-expressing Stable Cell Line

Name: Panoply™ Human PPM1K Over-expressing Stable Cell Line
SKU: CSC-SC012220
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC012220

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC012220
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PPM1K
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PPM1K protein phosphatase, Mg2+/Mn2+ dependent, 1K [ Homo sapiens ]
Gene Symbol	PPM1K
Synonyms	PPM1K; protein phosphatase, Mg2+/Mn2+ dependent, 1K; protein phosphatase 1K (PP2C domain containing); protein phosphatase 1K, mitochondrial; BDP; branched chain α ketoacid dehydrogenase phosphatase; DKFZp761G058; hPTMP; PP2C type mitochondrial phosphoprotein phosphatase; PP2Ckappa; PP2Cm; protein phosphatase 2C kappa; PP2C-kappa; PP2C-like protein; PP2C-like mitochondrial protein; PP2C type mitochondrial phosphatase; protein phosphatase 2C isoform kappa; PP2C domain-containing protein phosphatase 1K; PP2C-type mitochondrial phosphoprotein phosphatase; branched-chain and #945; -ketoacid dehydrogenase phosphatase; PTMP; UG0882E07; DKFZp667B084;
Gene ID	152926
Uni Prot ID	Q8N3J5
m RNA Refseq	BC037552
Chromosome Location	4q22.1
Function	hydrolase activity; metal ion binding; protein serine/threonine phosphatase activity;
MIM	611065

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