Panoply™ Human PPIA Over-expressing Stable Cell Line

Name: Panoply™ Human PPIA Over-expressing Stable Cell Line
SKU: CSC-SC012189
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC012189

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC012189
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PPIA
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PPIA peptidylprolyl isomerase A (cyclophilin A) [ Homo sapiens ]
Gene Symbol	PPIA
Synonyms	CYPA; CYPH
Gene Description	peptidylprolyl isomerase A (cyclophilin A)
GeneID	5478
Uni ProtID	P62937
mRNA Refseq	NM_021130.3
Protein Refseq	NP_066953.1
Chromosome Location	7p13
Function	peptide binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; unfolded protein binding; virion binding;
Pathway	APOBEC3G mediated resistance to HIV-1 infection, organism-specific biosystem; Basigin interactions, organism-specific biosystem; Binding and entry of HIV virion, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Disease, organism-specific biosystem; Early Phase of HIV Life Cycle, organism-specific biosystem; HIV Infection, organism-specific biosystem;
MIM	123840

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Researchers screened differentially expressed genes and alternative splicing genes in PPIA-overexpressing cells using transcriptome sequencing. The results identified 157 significantly upregulated genes and 171 significantly downregulated genes in PPIA-overexpressing cells, and altered splicing patterns were found in LHPP, APH1A, BRD1, and ORAI3. GO analysis showed that the most enriched GO entries for the 157 upregulated genes included extracellular regions, protein binding, and metal ions, while the most enriched GO entries for the 171 downregulated genes included neuronal projection binding, protein binding, and endoplasmic reticulum unfolded protein responses. Kyoto Encyclopedia of Genetics and Genomes (KEGG) analysis showed that the 157 upregulated genes were mainly enriched in gastric acid secretion and mitogen-activated protein kinase signaling pathways, while the 171 downregulated genes were mainly enriched in cancer transcriptional dysregulation and tumor necrosis factor signaling pathways. Overexpression of PPIA in human umbilical vein endothelial cells leads to altered expression of downstream genes and induces alternative splicing in multiple genes. PPIA induces vascular endothelial injury via CyPA-mediated high glucose by altering the expression or alternative splicing patterns of downstream genes.

The expression levels of nine differentially expressed genes (DEGs) were verified using real-time quantitative PCR. Compared with the control group, the expression levels of ATF4, PPP1R15A, GADD45B, SGK1, DDIT3, PMAIP1, SULF1, GDF6, and XBP1 were significantly reduced in PPIA-overexpressing cells, which was consistent with the sequencing results (Figure 1).

Figure 1. Real-time quantitative PCR validation of 9 differentially expressed genes. (Yang W, et al., 2024)

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