Panoply™ Human PLA2G12A Over-expressing Stable Cell Line

Name: Panoply™ Human PLA2G12A Over-expressing Stable Cell Line
SKU: CSC-SC011860
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC011860

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC011860
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PLA2G12A
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PLA2G12A phospholipase A2, group XIIA [ Homo sapiens ]
Gene Symbol	PLA2G12A
Synonyms	PLA2G12A; phospholipase A2, group XIIA; phospholipase A2, group XII , PLA2G12; group XIIA secretory phospholipase A2; sPLA2-XII; GXII sPLA2; group XII secreted phospholipase A2; group XIIA secreted phospholipase A2; phosphatidylcholine 2-acylhydrolase 12A; GXII; ROSSY; PLA2G12;
Gene ID	81579
Uni Prot ID	Q9BZM1
m RNA Refseq	BC017218
Chromosome Location	4q25
Function	calcium ion binding; calcium-dependent phospholipase A2 activity; hydrolase activity;
Pathway	Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem;
MIM	611652

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