Panoply™ Human PKMYT1 Over-expressing Stable Cell Line

Name: Panoply™ Human PKMYT1 Over-expressing Stable Cell Line
SKU: CSC-SC011849
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC011849

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC011849
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PKMYT1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PKMYT1 protein kinase, membrane associated tyrosine/threonine 1 [ Homo sapiens ]
Gene Symbol	PKMYT1
Synonyms	MYT1
GeneID	9088
Uni ProtID	B4DXD4
mRNA Refseq	NM_001258450.1
Protein Refseq	NP_001245379.1
Chromosome Location	16p13.3
Function	ATP binding; kinase activity; metal ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity;
Pathway	Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem; Cyclin E associated events during G1/S transition, organism-specific biosystem;
MIM	602474

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Overactivation of AKT drives the malignant phenotype of lung cancer by promoting tumor cell proliferation and survival. However, the relationship between dysregulated cell cycle progression and AKT1 kinase activity remains unclear. Here, researchers found that PKMYT1 is downregulated in lung adenocarcinoma (LUAD) tissues, and its low expression predicts poor prognosis in LUAD patients. PKMYT1 exerts a potent tumor-suppressive function in LUAD cells by inhibiting AKT1 activation and subsequently suppressing cell cycle progression, which depends on its tyrosine and threonine protein kinase activity. Interestingly, PKMYT1 can directly bind to AKT1, thereby inhibiting AKT1 activation. Furthermore, silencing AKT1 and using inhibitors targeting the AKT pathway effectively reversed the promoting effects of PKMYT1 knockdown on LUAD cell proliferation, migration, and invasion. These studies reveal the antitumor role of PKMYT1 in LUAD and provide evidence for elucidating the dual role of PKMYT1 in tumor progression.

Here, researchers investigated the function of PKMYT1 in lung adenocarcinoma (LUAD) cells. Overexpression of PKMYT1 significantly slowed the proliferation rate of A549 and H1299 cells, which have low basal expression levels of PKMYT1 (Figure 1a and 1b). Compared to the control group, PKMYT1-overexpressing A549 and H1299 cells formed fewer and smaller colonies in culture dishes and soft agar medium (Figure 1c and 1d). Furthermore, compared to control cells, PKMYT1-overexpressing A549 and H1299 cells showed reduced migration and invasion capabilities, with significantly fewer cells passing through the Transwell chambers and wider wound healing gaps (Figure 1e). Conversely, in PKMYT1-knockdown BEAS-2B cells, cell viability and migration ability were enhanced (Figure 1a-c and 1e). Simultaneously, silencing PKMYT1 promoted the proliferation (Figure 1a-c), anchorage-independent growth (Figure 1d), migration, and invasion (Figure 1e) of H1975 cells. In summary, PKMYT1 inhibits the malignant phenotype of LUAD cells in vitro.

Figure 1. PKMYT1 suppresses proliferation, growth, migration, and invasion of lung cancer cell lines in vitro. (Wang S, et al., 2023)

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