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Panoply™ Human PIGM Over-expressing Stable Cell Line

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-SC011762

Host Cell :   HEK293 (CHO and other cell types are also available) Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-SC011762
Description Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene PIGM
Gene Species Homo sapiens (Human)
Host Cell HEK293 (CHO and other cell types are also available)
Host Cell Species Species varies
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Disease research

Size 2 × 10^6 cells / vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry Ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name PIGM phosphatidylinositol glycan anchor biosynthesis, class M [ Homo sapiens ]
Gene Symbol PIGM
Synonyms GPI-MT-I
Gene Description phosphatidylinositol glycan anchor biosynthesis, class M
Gene ID 93183
Uni Prot ID Q9H3S5
m RNA Refseq NM_145167.2
Protein Refseq NP_660150.1
Chromosome Location 1q23.2
Function mannosyltransferase activity;
Pathway GPI-anchor biosynthesis, core oligosaccharide, organism-specific biosystem; GPI-anchor biosynthesis, core oligosaccharide, conserved biosystem; Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, organism-specific biosystem; Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, conserved biosystem; Metabolism of proteins, organism-specific biosystem; Post-translational modification: synthesis of GPI-anchored proteins, organism-specific biosystem; Post-translational protein modification, organism-specific biosystem;
MIM 610273
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