Panoply™ Human MMP7 Over-expressing Stable Cell Line

Name: Panoply™ Human MMP7 Over-expressing Stable Cell Line
SKU: CSC-SC009609
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC009609

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC009609
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	MMP7
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MMP7 matrix metallopeptidase 7 (matrilysin, uterine) [ Homo sapiens ]
Gene Symbol	MMP7
Synonyms	MMP-7; MPSL1; PUMP-1
Gene Description	matrix metallopeptidase 7 (matrilysin, uterine)
GeneID	4316
Uni ProtID	P09237
mRNA Refseq	NM_002423.3
Protein Refseq	NP_002414.1
Chromosome Location	11q21-q22
Function	metalloendopeptidase activity; zinc ion binding;
Pathway	Activation of Matrix Metalloproteinases, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Syndecan-1-mediated signaling events, organism-specific biosystem; Wnt Signaling Pathway and Pluripotency, organism-specific biosystem;
MIM	178990

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Matrix metalloproteinase 7 (MMP7), the smallest member of the matrix metalloproteinase family, has been shown to be closely associated with cancer progression, especially metastasis. However, its expression pattern and function in tongue cancer remain unclear. Here, researchers found that MMP7 expression was significantly increased in human tongue squamous cell carcinoma (TSCC) specimens compared to paired non-tumor tissues, and this high expression was closely associated with lymph node metastasis in patients. Furthermore, molecular functional assays confirmed that MMP7 promotes the proliferation, migration, and invasion of TSCC cells. Knockdown of MMP7 inhibited lymph node metastasis in vivo. Therefore, MMP7 plays an oncogenic role in the occurrence and metastasis of tongue cancer and may be a potential therapeutic target for tongue cancer.

Here, researchers constructed MMP7-overexpressing CAL27 and SCC9 cells and validated the results using real-time quantitative PCR (Figure 1a) and Western blotting (Figure 1b). The results of the CCK8 assay (Figure 1c) and clonogenic assay (Figure 1d) showed that MMP7 overexpression accelerated the proliferation process of CAL27 and SCC9 cells.

Figure 1. Overexpression of MMP7 promotes tongue cancer cell line growth in vitro. (Yuan S, et al., 2020)

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