Panoply™ Human MCCC1 Over-expressing Stable Cell Line

Name: Panoply™ Human MCCC1 Over-expressing Stable Cell Line
SKU: CSC-SC009302
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC009302

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC009302
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	MCCC1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MCCC1 methylcrotonoyl-CoA carboxylase 1 (alpha) [ Homo sapiens ]
Gene Symbol	MCCC1
Synonyms	MCCA; MCC-B
Gene Description	methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
Gene ID	56922
Uni Prot ID	Q96RQ3
m RNA Refseq	NM_020166.3
Protein Refseq	NP_064551.3
Chromosome Location	3q27
Function	ATP binding; biotin binding; biotin carboxylase activity; metal ion binding; methylcrotonoyl-CoA carboxylase activity;
Pathway	Branched-chain amino acid catabolism, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem;
MIM	609010

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