Panoply™ Human KDM1A Over-expressing Stable Cell Line

Name: Panoply™ Human KDM1A Over-expressing Stable Cell Line
SKU: CSC-SC008039
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC008039

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC008039
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	KDM1A
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KDM1A lysine (K)-specific demethylase 1A [ Homo sapiens ]
Gene Symbol	AOF2
Synonyms	AOF2; KDM1; LSD1; BHC110
Gene Description	amine oxidase (flavin containing) domain 2
Gene ID	23028
Uni Prot ID	O60341
m RNA Refseq	NM_001009999.2
Protein Refseq	NP_001009999.1
Chromosome Location	1p36.12
Function	MRF binding; RNA polymerase II transcription factor binding; androgen receptor binding; chromatin binding; demethylase activity; enzyme binding; flavin adenine dinucleotide binding; histone demethylase activity; histone demethylase activity (H3-K4 specific); histone demethylase activity (H3-K9 specific); histone demethylase activity (H3-dimethyl-K4 specific); ligand-dependent nuclear receptor transcription coactivator activity; oxidoreductase activity; p53 binding; protein binding; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding;
Pathway	Coregulation of Androgen receptor activity, organism-specific biosystem; Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Hemostasis, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem;
MIM	609132

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Here, researchers explored the biological function and mechanisms of KDM1A in epithelial-mesenchymal transition (EMT) in gastric cancer. The study found that KDM1A is highly expressed in gastric cancer tumor tissue. In clinical gastric cancer samples, KDM1A expression levels were negatively correlated with E-cadherin expression and positively correlated with vimentin expression. In gastric cancer patients, KDM1A expression was associated with lymph node metastasis, TNM staging, and tumor differentiation. Downregulation of KDM1A expression inhibited cell migration, invasion, and F-actin expression, and suppressed the EMT process. Overexpression of KDM1A promoted EMT in gastric cancer cells. KDM1A affects EMT in gastric cancer cells by regulating the TGF-β/Notch signaling pathway.

Here, researchers studied the morphology and F-actin expression of KDM1A-knockdown and KDM1A-overexpressing HGC-27 and AGS cells. As shown in Figure 1A, after KDM1A overexpression, HGC-27 and AGS cells exhibited a morphology similar to mesenchymal cells, mostly elongated and spindle-shaped. However, after KDM1A knockdown, most HGC-27 and AGS cells were flattened and polygonal. Fluorescently labeled phalloidin staining showed that KDM1A-overexpressing HGC-27 and AGS cells had more F-actin staining around the cytoplasm, and prominent pseudopods were observed (Figure 1B). In KDM1A-knockdown cells, there was less F-actin accumulation, and no prominent pseudopods were observed (Figure 1B).

Figure 1. KDM1A affects the morphology and F‐actin expression of gastric cancer cells. (Huang R, et al., 2025)

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