Panoply™ Human IL22 Over-expressing Stable Cell Line

Name: Panoply™ Human IL22 Over-expressing Stable Cell Line
SKU: CSC-SC007576
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC007576

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC007576
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	IL22
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	IL22 interleukin 22 [ Homo sapiens ]
Gene Symbol	IL22
Synonyms	IL22; interleukin 22; interleukin-22; IL 10 related T cell derived inducible factor; IL 21; IL 22; IL D110; IL TIF; ILTIF; MGC79382; MGC79384; TIFa; TIFIL 23; zcyto18; cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-21; IL-22; IL-TIF; IL-D110; TIFIL-23;
GeneID	50616
Uni ProtID	Q9GZX6
mRNA Refseq	BC069308
Chromosome Location	12q15
Function	cytokine activity; interleukin-22 receptor binding;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
MIM	605330

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Inflammation is considered a hallmark of cancer and is closely associated with gastric cancer. Interleukin-22 (IL-22), a member of the IL-10 family, plays a crucial role in inflammatory diseases and tumors. In this study, researchers investigated the effect of IL-22 on the proliferation of cultured gastric cancer cells (AGS cells) and the underlying molecular mechanisms. Cell Counting Kit-8 (CCK-8) assays using AGS cells transfected with an IL-22 plasmid demonstrated that IL-22 promoted cell viability; conversely, knockdown of IL-22 expression using short hairpin RNA (shRNA) significantly reduced AGS cell viability. Western blotting results revealed that IL-22 inhibited the activation of the mitogen-activated protein kinase (MAPK) signaling pathway. Furthermore, transfection with IL-22-shRNA enhanced MAPK pathway activation, as evidenced by the upregulation of ERK and JNK phosphorylation levels. Collectively, these findings indicate that IL-22 regulates gastric cancer cell viability via the JNK signaling pathway, suggesting that targeting IL-22 may represent a potential therapeutic strategy for gastric cancer.

Here, the viability of AGS cells was assessed using the CCK-8 assay at various time points following transfection. As shown in Figure 1, IL-22-overexpressing AGS cells showed significantly increased viability compared with the control group. Conversely, IL-22 knockdown significantly reduced the viability of AGS cells compared to the control shRNA group.

Figure 1. IL-22 increases the viability of AGS cells. (Dong H, et al., 2020)

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