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Panoply™ Human HIF3A Over-expressing Stable Cell Line

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-SC006989

Host Cell :   HEK293 (CHO and other cell types are also available) Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No. CSC-SC006989
Description Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene HIF3A
Gene Species Homo sapiens (Human)
Host Cell HEK293 (CHO and other cell types are also available)
Host Cell Species Species varies
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Disease research

Size 2 × 10^6 cells / vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry Ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name HIF3A hypoxia inducible factor 3, alpha subunit [ Homo sapiens ]
Gene Symbol HIF3A
Synonyms HIF3A; hypoxia inducible factor 3, alpha subunit; hypoxia-inducible factor 3-alpha; bHLHe17; IPAS; MOP7; PASD7; HIF3-alpha-1; member of PAS protein 7; inhibitory PAS domain protein; PAS domain-containing protein 7; basic-helix-loop-helix-PAS protein MOP7; class E basic helix-loop-helix protein 17; HIF-3A;
Gene ID 64344
Uni Prot ID Q9Y2N7
m RNA Refseq BC080551
Chromosome Location 19q13
Function DNA binding; sequence-specific DNA binding transcription factor activity; signal transducer activity;
Pathway Hypoxic and oxygen homeostasis regulation of HIF-1-alpha, organism-specific biosystem;
MIM 609976
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