Panoply™ Human HDAC8 Over-expressing Stable Cell Line

Name: Panoply™ Human HDAC8 Over-expressing Stable Cell Line
SKU: CSC-SC006893
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC006893

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC006893
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	HDAC8
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HDAC8 histone deacetylase 8 [ Homo sapiens ]
Gene Symbol	HDAC8
Synonyms	HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
Gene Description	histone deacetylase 8
Gene ID	55869
Uni Prot ID	Q9BY41
m RNA Refseq	NM_001166418.1
Protein Refseq	NP_001159890.1
Chromosome Location	Xq13
Function	NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K18 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; metal ion binding; transcription factor binding;
Pathway	Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Cell cycle, organism-specific biosystem; Integrated Pancreatic Cancer Pathway, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Neural Crest Differentiation, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
MIM	300269

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Macroautophagy/autophagy has been exploited by many viruses, including foot-and-mouth disease virus (FMDV), to promote viral replication, but the underlying mechanisms of the interaction between autophagy and the innate immune response remain unclear. Here, researchers show that histone deacetylase 8 (HDAC8) inhibits FMDV replication by regulating innate immune signaling and antiviral responses. To counteract the effect of HDAC8, FMDV utilizes autophagy to promote HDAC8 degradation. Further data revealed that the FMDV structural protein VP3 promotes autophagy during viral infection and interacts with and degrades HDAC8 through an AKT-MTOR-ATG5-dependent autophagy pathway. These studies demonstrate that FMDV has evolved a strategy to counteract the host's antiviral activity by degrading proteins that regulate the innate immune response during viral infection through autophagy.

Innate immunity is the first line of defense against viral infections. To investigate the function of HDAC8 in regulating immune responses, researchers examined the expression of interferon-related genes using control and HDAC8 gene knockout cell lines. As shown in Figure 1A, they detected the expression of FMDV structural proteins using FMDV polyclonal antibodies. Compared to control cells, knocking out HDAC8 in the PK-15 cell line significantly reduced the phosphorylation levels of TBK1 and IRF3 during FMDV infection, which are key regulators of interferon production and subsequent immune responses, leading to increased viral replication in the knockout cells. Furthermore, the phosphorylation levels of TBK1 and IRF3 were significantly enhanced during FMDV infection in HDAC8-overexpressing PK-15 cells, thereby attenuating viral replication (Figure 1B). Real-time quantitative PCR results also showed that the expression levels of IFNB, IFIT2/ISG54, CCL5, OAS, and TNF were significantly reduced in HDAC8 knockout cells compared to control cells, while they were significantly increased in HDAC8-overexpressing cells (Figure 1C, D). These data consistently indicate that HDAC8 regulates the innate immune response during FMDV infection.

Figure 1. HDAC8 involves in the antiviral signaling pathway. (Zhang H, et al., 2023)

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