Panoply™ Human HDAC3 Over-expressing Stable Cell Line

Name: Panoply™ Human HDAC3 Over-expressing Stable Cell Line
SKU: CSC-SC006888
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC006888

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC006888
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	HDAC3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HDAC3 histone deacetylase 3 [ Homo sapiens ]
Gene Symbol	HDAC3
Synonyms	HD3; RPD3; RPD3-2
Gene Description	histone deacetylase 3
Gene ID	8841
Uni Prot ID	O15379
m RNA Refseq	NM_003883.3
Protein Refseq	NP_003874.2
Chromosome Location	5q31
Function	NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K18 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin DNA binding; enzyme binding; histone deacetylase activity; histone deacetylase activity; histone deacetylase binding; protein binding; protein deacetylase activity; transcription corepressor activity; transcription factor binding;
Pathway	Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Cell cycle, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Developmental Biology, organism-specific biosystem;
MIM	605166

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FOXA1 is associated with malignant tumors, but its function in epithelial ovarian carcinoma (EOC) remains unclear. HDAC3 can affect the proliferation, migration, and invasion capabilities of EOC cells. This study aimed to investigate the function of FOXA1 in ovarian cancer and the relationship between HDAC3 and FOXA1. Immunohistochemical staining was performed on primary lesions from 127 patients with ovarian epithelial cancer to detect the expression of HDAC3 and FOXA1. Cell proliferation, migration, and apoptosis were assessed using proliferation assays, Transwell assays, apoptosis assays, and animal experiments before and after FOXA1 transfection. In FIGO stage III-IV ovarian cancer patients, the staining H-scores of FOXA1 and HDAC3 were significantly increased and predicted poor clinical prognosis. The expression level of HDAC3 was significantly correlated with the expression level of FOXA1. In FOXA1 knockdown cells, the invasion, proliferation, and apoptosis capabilities, as well as tumor formation ability, were reduced. Xenograft experiments confirmed that HDAC3 mediates tumor formation. In conclusion, FOXA1 can be regulated by HDAC3 through the Wnt/β-catenin signaling pathway, and FOXA1 plays an important role in the proliferation, apoptosis, and invasion processes in EOC cell lines and xenograft experiments.

To determine the correlation between HDAC3 and FOXA1 expression, researchers performed Western blot experiments. RT-qPCR and Western blot analysis were used to detect the transfection efficiency after transfecting SKOV3 and ES-2 cells with the HDAC3 lentiviral vector (Figure 1A). Western blot results showed that FOXA1 expression levels were significantly decreased in HDAC3 knockdown cells, while they were significantly increased in HDAC3 overexpressing cells. Compared to the corresponding control groups, the expression levels of β-catenin, cyclin D1, and MMP2 were decreased in HDAC3 knockdown cells and significantly increased in HDAC3 overexpressing cells (Figure 1B).

Figure 1. HDAC3 affects FOXA1 and the Wnt/β-catenin signaling pathway. (Lou T, et al., 2022)

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