Panoply™ Human GRB14 Over-expressing Stable Cell Line

Name: Panoply™ Human GRB14 Over-expressing Stable Cell Line
SKU: CSC-SC006635
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC006635

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC006635
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	GRB14
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GRB14 growth factor receptor-bound protein 14 [ Homo sapiens ]
Gene Symbol	GRB14
Gene Description	growth factor receptor-bound protein 14
Gene ID	2888
Uni Prot ID	Q14449
m RNA Refseq	NM_004490.2
Protein Refseq	NP_004481.2
Chromosome Location	2q22-q24
Function	SH3/SH2 adaptor activity; phospholipid binding; phosphoprotein binding;
Pathway	Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Hemostasis, organism-specific biosystem; Insulin Pathway, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Tie2 Signaling, organism-specific biosystem;
MIM	601524

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