Panoply™ Human ENPP3 Over-expressing Stable Cell Line

Name: Panoply™ Human ENPP3 Over-expressing Stable Cell Line
SKU: CSC-SC004948
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC004948

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC004948
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	ENPP3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ENPP3 ectonucleotide pyrophosphatase/phosphodiesterase 3 [ Homo sapiens ]
Gene Symbol	ENPP3
Synonyms	B10; NPP3; PDNP3; CD203c; PD-IBETA
Gene Description	ectonucleotide pyrophosphatase/phosphodiesterase 3
GeneID	5169
Uni ProtID	O14638
mRNA Refseq	NM_005021.3
Protein Refseq	NP_005012.2
Chromosome Location	6q22
Function	NADH pyrophosphatase activity; metal ion binding; nucleic acid binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; scavenger receptor activity;
Pathway	Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Pantothenate and CoA biosynthesis, organism-specific biosystem; Pantothenate and CoA biosynthesis, conserved biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Riboflavin metabolism, organism-specific biosystem;
MIM	602182

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Mounting evidence suggests that aberrant methylation plays a crucial role in the development and progression of endometriosis (EMs). Here, researchers investigated the relationship between aberrant ENPP3 promoter methylation and the pathogenesis of ovarian EMs, focusing on its regulatory role on ENPP3 expression. Results showed that the methylation level of the CpG site in the ENPP3 promoter region was significantly reduced in ectopic endometrial tissue compared to normal endometrial tissue. RT-qPCR results revealed significantly increased ENPP3 expression in ovarian EMs tissue compared to endometrial tissue from patients without EMs, and this upregulation was negatively correlated with the methylation level of the ENPP3 promoter region. Immunohistochemical analysis confirmed increased ENPP3 expression in glandular epithelial cells and stroma of ovarian EMs tissue. Furthermore, in vitro experiments showed that overexpression of ENPP3 significantly enhanced the invasion and migration capabilities of endometrial stromal cells. Transcriptome sequencing and functional analysis indicated that increased ENPP3 expression activated the AKT/mTOR/4EBP1 signaling pathway. In summary, this study suggests that hypomethylation of the ENPP3 promoter region may promote the occurrence and development of ovarian endometriosis by activating the AKT/mTOR/4EBP1 pathway, supporting the theory that endometriosis may be an epigenetically regulated disease.

Transwell chamber migration assay was used to evaluate the migration and invasion abilities of cells. The results showed that the migration and invasion abilities of ENPP3-overexpressing human endometrial stromal cells (HESCs) were significantly enhanced (Figure 1).

Figure 1. Overexpression of ENPP3 promoted cell migratory and invasive capabilities. (Qin Y, et al., 2024)

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