Panoply™ Human ENPP2 Knockdown Stable Cell Line

Name: Panoply™ Human ENPP2 Knockdown Stable Cell Line
SKU: CSC-DC004947
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC004947

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

Inquire for Price

Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC004947
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	ENPP2
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ENPP2 ectonucleotide pyrophosphatase/phosphodiesterase 2 [ Homo sapiens ]
Gene Symbol	ENPP2
Synonyms	ENPP2; ectonucleotide pyrophosphatase/phosphodiesterase 2; PDNP2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ATX; autotaxin; PD IALPHA; E-NPP 2; autotaxin-t; plasma lysophospholipase D; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2; NPP2; ATX-X; LysoPLD; AUTOTAXIN; PD-IALPHA; FLJ26803;
Gene ID	5168
Uni Prot ID	Q13822
m RNA Refseq	BC034961
Chromosome Location	8q24.1
Function	alkylglycerophosphoethanolamine phosphodiesterase activity; calcium ion binding; hydrolase activity; lysophospholipase activity; nucleic acid binding; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; scavenger receptor activity; transcription factor binding; zinc ion binding;
Pathway	Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem;
MIM	601060

Quick Inquiry

Case Study

Q & A

Customer Reviews

Lipid metabolism disorders are a key factor in the progression of chronic lymphocytic leukemia (CLL). Here, researchers identified significant differences in the levels of 52 lipids between CLL samples and healthy controls. Functional analysis showed that alterations in glycerol ester metabolism, glycerophospholipid metabolism, sphingolipid metabolism, and related metabolic pathways had the greatest impact on CLL. Based on the area under the curve (AUC), combinations of three metabolites (phosphatidylcholine O-24:2-18:2, phosphatidylcholine O-35:3, and lysophosphatidylcholine 34:3) hold promise as biomarkers for CLL diagnosis. Furthermore, using integrated lipidomics, transcriptomics, and molecular biology studies, researchers revealed that exonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) plays a crucial role in regulating carcinogenic lipogenesis. ENPP2 expression was significantly elevated in CLL patients compared to normal cells, a finding validated in an independent cohort. Furthermore, ENPP2 knockdown and treatment with the targeted inhibitor PF-8380 exert anti-tumor effects by regulating cell viability, proliferation, apoptosis, and cell cycle, and enhance cellular sensitivity to ibrutinib. Mechanistically, ENPP2 promotes adipogenesis by inhibiting phosphorylation of AMP-activated protein kinase (AMPK) and by binding to the transcription factor 1 (SREBP-1)/fatty acid synthase (FAS) signaling pathway via sterol regulatory elements.

To validate the results of the bioinformatics analysis, researchers conducted functional experiments in chronic lymphocytic leukemia (CLL) cells to investigate the role of ENPP2. ENPP2 was successfully silenced in MEC-1 and EHEB cells (Figure 1A). They confirmed through a CCK-8 assay that downregulation of ENPP2 indirectly inhibited CLL cell proliferation (Figure 1B). Furthermore, Annexin V-PE/7AAD assays showed a significant increase in apoptosis in ENPP2-knockdown cells (Figures 1C-E). Additionally, cell cycle monitoring of ENPP2-knockdown cells revealed significant G0/G1 phase arrest compared to control cells (Figures 1F-I). These results indicate that ENPP2 plays a crucial role in CLL cell survival by inhibiting apoptosis and promoting cell progression from the G0/G1 phase.

Figure 1. ENPP2 knockdown restrained the survival of CLL cell lines. (Lu L, et al., 2024)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *