Panoply™ Human DGKE Over-expressing Stable Cell Line

Name: Panoply™ Human DGKE Over-expressing Stable Cell Line
SKU: CSC-SC004253
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC004253

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC004253
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	DGKE
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DGKE diacylglycerol kinase, epsilon 64kDa [ Homo sapiens ]
Gene Symbol	DGKE
Synonyms	DGKE; diacylglycerol kinase, epsilon 64kDa; diacylglycerol kinase, epsilon (64kD); diacylglycerol kinase epsilon; DAGK6; DGK; DGK-epsilon; DAG kinase epsilon; diglyceride kinase epsilon; DAGK5;
Gene ID	8526
Uni Prot ID	P52429
m RNA Refseq	BC130629
Chromosome Location	17q22
Function	ATP binding; diacylglycerol kinase activity; metal ion binding; nucleotide binding; transferase activity;
Pathway	Effects of PIP2 hydrolysis, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem;
MIM	601440

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