Panoply™ Human DAO Over-expressing Stable Cell Line

Name: Panoply™ Human DAO Over-expressing Stable Cell Line
SKU: CSC-SC004020
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC004020

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC004020
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	DAO
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DAO D-amino-acid oxidase [ Homo sapiens ]
Gene Symbol	DAO
Synonyms	DAO; D-amino-acid oxidase; DAMOX; D-amino acid oxidase; DAAO; OXDA; MGC35381;
Gene ID	1610
Uni Prot ID	P14920
m RNA Refseq	BC029057
Chromosome Location	12
Function	D-amino-acid oxidase activity; D-amino-acid oxidase activity; FAD binding; nucleotide binding; oxidoreductase activity; protein binding; protein dimerization activity;
Pathway	Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; D-Arginine and D-ornithine metabolism, organism-specific biosystem; D-Arginine and D-ornithine metabolism, conserved biosystem; Glycine, serine and threonine metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, conserved biosystem; Glyoxylate metabolism, organism-specific biosystem;
MIM	124050

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Patient-derived organoids provide a powerful platform for elucidating drug resistance mechanisms and tumor evolution in hepatocellular carcinoma (HCC), as well as identifying novel therapeutic targets. Here, researchers found that DAO expression was significantly downregulated in successfully constructed HCC organoids compared to those from failed organoid construction. DAO expression was also significantly downregulated in sorafenib-resistant organoids compared to parental tissues. In the TCGA-LIHC cohort, DAO expression was significantly reduced in advanced HCC tissues and negatively correlated with stem cell characteristics and epithelial-mesenchymal transition (EMT)-related molecules. Reduced DAO expression was associated with poorer overall survival in HCC patients. In HepG2 cells, DAO knockdown significantly enhanced cell proliferation. Ectopic DAO expression inhibited the proliferation, migration, and invasion of HepG2 and SK-Hep-1 cells. Supplementation with D-alanine (D-Ala) further enhanced the antiproliferative effect of DAO overexpression, but did not significantly alter DAO-mediated migration or invasion inhibition. Ectopic DAO expression induced apoptosis by generating H₂O₂ through simultaneous D-Ala supplementation in the culture medium. The addition of catalase (an H₂O₂ degrading enzyme) significantly reversed D-Ala-induced apoptosis. In the BALB/c nude mouse model, hepatocellular carcinoma (HCC) cells overexpressing DAO formed significantly smaller tumors than control cells, and D-alanine supplementation further enhanced this anti-tumor effect. Ectopic DAO expression restored the sensitivity of drug-resistant organoids to sorafenib.

To elucidate the role of DAO in hepatocellular carcinoma (HCC), researchers investigated the effects of DAO on the in vitro growth, migration, and invasion of HepG2 and SK-Hep-1 cell lines, as well as HCC118-SR and HCC25-SR human HCC organoids. Compared to the control group (HepG2-siControl), the DAO knockdown group (HepG2-siDAO) significantly enhanced cell proliferation. Furthermore, when D-Ala was added to HepG2-siDAO cells (HepG2-siDAO+D-Ala), there was no significant change in cell proliferation rate compared to HepG2-siDAO alone (Figure 1a). CCK8 assays showed that DAO-overexpressing HepG2 and SK-Hep-1 cells significantly reduced cell proliferation, and the addition of D-Ala further enhanced this inhibitory effect (Figures 1b and 1c). MTT assays showed that ectopic expression of DAO in HCC118-SR and HCC25-SR organoids significantly reduced cell proliferation (Figure 1d and e). Flow cytometry analysis revealed a significant decrease in the proportion of S-phase cells in DAO-overexpressing HepG2 and SK-Hep-1 cells. These results further indicate that DAO inhibits HCC cell proliferation (Figure 1f and g).

Figure 1. The effect of DAO on proliferation and cell cycle. (Li Z, et al., 2025)

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