Panoply™ Human CYP2C19 Over-expressing Stable Cell Line

Name: Panoply™ Human CYP2C19 Over-expressing Stable Cell Line
SKU: CSC-SC003957
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC003957

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC003957
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	CYP2C19
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CYP2C19 cytochrome P450, family 2, subfamily C, polypeptide 19 [ Homo sapiens ]
Gene Symbol	CYP2C19
Synonyms	CPCJ; CYP2C; P450C2C; P450IIC19
Gene Description	cytochrome P450, family 2, subfamily C, polypeptide 19
Gene ID	1557
Uni Prot ID	P33261
m RNA Refseq	NM_000769.1
Protein Refseq	NP_000760.1
Chromosome Location	10q24
Function	(R)-limonene 6-monooxygenase activity; (S)-limonene 6-monooxygenase activity; (S)-limonene 7-monooxygenase activity; electron carrier activity; enzyme binding; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; oxygen binding; steroid hydroxylase activity;
Pathway	Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Biological oxidations, organism-specific biosystem; Chemical carcinogenesis, organism-specific biosystem; Chemical carcinogenesis, conserved biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem;
MIM	124020

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