Panoply™ Human CHEK1 Over-expressing Stable Cell Line

Name: Panoply™ Human CHEK1 Over-expressing Stable Cell Line
SKU: CSC-SC003082
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC003082

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC003082
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	CHEK1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHEK1 checkpoint kinase 1 [ Homo sapiens ]
Gene Symbol	CHEK1
Synonyms	CHK1
Gene ID	1111
Uni Prot ID	B4DT73
m RNA Refseq	NM_001114121.2
Protein Refseq	NP_001107593.1
Chromosome Location	11q24.2
Function	ATP binding; histone kinase activity (H3-T11 specific); protein binding; protein serine/threonine kinase activity;
Pathway	Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex, organism-specific biosystem;
MIM	603078

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Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer-related death. This study aimed to investigate the role of checkpoint kinase 1 (CHEK1) in NSCLC progression and its regulatory relationship with forkhead box protein M1 (FOXM1). The results showed that both FOXM1 and CHEK1 are highly expressed in NSCLC tissues. CHEK1 overexpression promotes NSCLC cell proliferation, while knockdown of CHEK1 inhibits cell proliferation, suppresses epithelial-mesenchymal transition (EMT), and reduces tumor growth in vivo. FOXM1 can directly bind to the CHEK1 promoter, thereby upregulating CHEK1 expression. These findings suggest that CHEK1 and FOXM1 may be potential therapeutic targets for NSCLC.

To investigate whether CHEK1 affects the progression of non-small cell lung cancer (NSCLC), researchers first constructed A549 and H1299 cell lines stably overexpressing CHEK1 (Figures 1A and B). They then examined the expression levels of epithelial-mesenchymal transition (EMT) markers (N-cadherin, E-cadherin, and vimentin) in these two CHEK1-overexpressing cell lines. The results showed that, compared to the control group, N-cadherin was significantly upregulated and E-cadherin was significantly downregulated in CHEK1-overexpressing A549 cells. Compared to the control group, both N-cadherin and vimentin were significantly upregulated in CHEK1-overexpressing H1299 cells (Figures 1C and D). Furthermore, the researchers used Transwell assays to examine the effect of stable CHEK1 overexpression on the migration and invasion abilities of A549 and H1299 cells. As shown in Figures 1E and 1F, the migration and invasion abilities of CHEK1-overexpressing A549 and H1299 cells were significantly enhanced. Next, to investigate whether CHEK1 overexpression regulates the proliferation of non-small cell lung cancer (NSCLC) cells, they assessed the effect of stable CHEK1 overexpression on the survival of A549 and H1299 cells. The results showed that the survival of both CHEK1-overexpressing cell lines was significantly improved (Figure 1G and H). Furthermore, stable CHEK1 overexpression significantly reduced the proportion of cells in S phase (Figure 1I-L).

Figure 1. CHEK1 overexpression promotes the migration and proliferation of NSCLC cells. (Lu X, et al., 2025)

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