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Panoply™ Human ARFGAP1 Over-expressing Stable Cell Line

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-SC000834

Host Cell :   HEK293 (CHO and other cell types are also available) Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No. CSC-SC000834
Description Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene ARFGAP1
Gene Species Homo sapiens (Human)
Host Cell HEK293 (CHO and other cell types are also available)
Host Cell Species Species varies
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Disease research

Size 2 × 10^6 cells / vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry Ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name ARFGAP1 ADP-ribosylation factor GTPase activating protein 1 [ Homo sapiens ]
Gene Symbol ARFGAP1
Synonyms ARF1GAP; HRIHFB2281
Gene Description ADP-ribosylation factor GTPase activating protein 1
Gene ID 55738
Uni Prot ID Q8N6T3
m RNA Refseq NM_018209.2
Protein Refseq NP_060679.1
Chromosome Location 20q13.33
Function ARF GTPase activator activity; GTPase activator activity; zinc ion binding;
Pathway Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Arf1 pathway, organism-specific biosystem; COPI Mediated Transport, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Endocytosis, organism-specific biosystem;
MIM 608377
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