Panoply™ Human AKT2 Over-expressing Stable Cell Line

Name: Panoply™ Human AKT2 Over-expressing Stable Cell Line
SKU: CSC-SC000466
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC000466

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC000466
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	AKT2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	AKT2 v-akt murine thymoma viral oncogene homolog 2 [ Homo sapiens ]
Gene Symbol	AKT2
Synonyms	PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Gene Description	v-akt murine thymoma viral oncogene homolog 2
Gene ID	208
Uni Prot ID	B4DG79
m RNA Refseq	NM_001243027.1
Protein Refseq	NP_001229956.1
Chromosome Location	19q13.1-q13.2
Function	ATP binding; kinase activity; phospholipid binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity;
Pathway	AKT phosphorylates targets in the cytosol, organism-specific biosystem; AKT phosphorylates targets in the nucleus, organism-specific biosystem; AKT-mediated inactivation of FOXO1A, organism-specific biosystem; AMPK signaling, organism-specific biosystem; Activation of PKB, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem;
MIM	164731

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