Panoply™ Human ACTN2 Over-expressing Stable Cell Line

Name: Panoply™ Human ACTN2 Over-expressing Stable Cell Line
SKU: CSC-SC000198
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC000198

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC000198
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	ACTN2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ACTN2 actinin, alpha 2 [ Homo sapiens ]
Gene Symbol	ACTN2
Synonyms	CMD1AA
Gene Description	actinin, alpha 2
Gene ID	88
Uni Prot ID	P35609
m RNA Refseq	NM_001103.2
Protein Refseq	NP_001094.1
Chromosome Location	1q42-q43
Function	FATZ binding; actin filament binding; calcium ion binding; cytoskeletal protein binding; identical protein binding; integrin binding; ion channel binding; protein binding; protein dimerization activity; structural constituent of muscle; thyroid hormone receptor coactivator activity; titin Z domain binding; titin binding;
Pathway	Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Arrhythmogenic right ventricular cardiomyopathy, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem;
MIM	102573

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